by Scott Olesen and Claire Duvallet
swo at alum dot mit dot edu
Distribution-based OTU calling (dbOTU, also known as
improves on other
approaches to calling operational taxonomic units (OTUs) by incorporating
information about the input sequences' distribution across samples. Previous
implementations of dbOTU presented challenges for users. dbOTU3 is a new
implementation of dbOTU that is faster and more user-friendly.
The theoretical and practical improvements over previous implementations of
dbOTU are described in this paper:
Scott W. Olesen, Claire Duvallet, and Eric J. Alm.
2017. dbOTU3: A new implementation of distribution-based OTU calling.
PLoS ONE 12(5): e0176335. doi: 10.1371/journal.pone.0176335
The original dbOTU algorithm was developed by Sarah Preheim and used to process Illumina Genome
Analyzer II reads for 16S rRNA sequence analysis in this paper:
Sarah P. Preheim, Allison R. Perrotta, Antonio M. Martin-Platero, Anika Gupta and Eric J. Alm. 2013.
Distribution-based clustering: Using ecology to refine the operational taxonomic unit. Appl. Environ. Microbiol. 2013, 79(21):659